Lab Report on Enzyme Reactions

Lab report April 14, 2013 Abstract In this article, we exit experiment on the significant in long suit of the enzyme by using three divergent test tubes and measuring the arrive of product they give off. To determine this we are going to test the join of color absorbance by using a special tool to help us guess our way outs. We will see how our block results base the effect of the amount of submersion we apply to each test tube. The results would be shown by the support of two graphs. IntroductionEnzymes are highly important because of the significant role they bunk in living things and animals. They are a factor which allows whether or not a reply occurs. To take place out the amount of strength an enzyme has, we need to first understand our outcome. In this experiment, our products will determine the strength of our enzymes by measuring the amount of color change. We must understand the measurement of the appearing product fashion in the experiment. We will determine t his by using a special equipment to help us understand our result better.In this study we will see how a specific enzyme could vary based on the contrastive types of factors that affect it. The question we are going to be addressing in this study is, will the amount of enzyme concentration affect a reaction? Materials and Methods The materials used in the making of this experiment are as say Three test tubes, 10. 5mL of distilled water, 6mL of Catechol, 1. 5mL of Extract(potato), a Spectronic 20 (spectrophotometer), Wax draw, a Test tube rack, three Parafilm, tissues to clear-cut the Spectronic 20 and the test tubes, 3 pipets and a timer.So we wont confuse ourselves, we used the wax pencil to label each test tube as we placed the requisite amount of substance in each of them. To avoid any slight errors, we also used three different pipets for the three required materials, which are the distilled water, catechol and the extract, respectively. Its important to take the precise m easurement so there wont be any errors in doing the experiment. Its also important that the extract goes last due to the reaction occurring in a timed pace. ResultsAccording to the graph shown above, we see our lay out of the experiment cosmos done. We can see how the materials are being distributed in each test tube according to the amount of ingredients required. This is highly crucial because a slight mistake can impact the experiment being done. Our end result is as follows According to the experiment done right away, we see that in test tube two, it show the most absorbance or color change. This shows that the enzymes strength was stronger than the rest, resulting into having more reaction and color change among the others.Discussion As stated before, the question we had was, will the amount of enzyme concentration affect a reaction? The end result was yes, the amount of enzyme concentrated inserted into each test tube did have a significant affect to the reaction by the amou nt of products it gave off (color strength). We used the extract (enzyme) as our independent variable because this is what we are testing. In contrast, we used the amount of absorbance as our dependent variable.We also made sure that our control done in this experiment was the catechol. As predicted, we were able to have more enzyme reaction in one of the test tubes than the others. The noesis we gain today was how much the amount of concentration enzyme affects the other ingredients. We see that our experiment done today can further lead into more research as in how enzymes are used to quick an action. Its also important because we should know the reason why and how enzymes reactions occur at a dissolute rate.